Enteropeptidase; Recombinant; Light Chain; 9014-74-8; Enterokinase

• Customization: Available
• CAS No.: 9014-74-8
• Formula: /
• Model NO.: YQEN0009
• Classification: Biochemical Reagents
• Grade: Br
• Specific Usage: For Biological Purpose, Technical Grade, Pratical Use
• Content: 95%
• Usage: Laboratory Reagents, Teaching Reagents
• Source: Recombinant
• Habit Appellation: Enterokinase
• Application: Industry, Agriculture
• Property: Biochemical Reagent
• Appearance: White or off White Powder
• Store: -20
• Solubility: Dissolve in Water
• Type: Protease
• Transport Package: Plastic Bottle
• Specification: 100u; 250u; 500u
• Trademark: /
• Origin: China
• Production Capacity: 90000pieces/Year

Product Description

Product Description

 

Product Name	Enteropeptidase
CAS	9014-74-8
Purity (content)	Recombinant
Code	YQEN0009
Packing	100u; 250u; 500u

High purity and high activity enterokinase expressed in Escherichia coli through refolding production

Product Introduction:
Escherichia coli expresses recombinant highly active and high-purity bovine enterokinase produced by in vitro refolding. This product is a highly specific proteolytic enzyme with specific cleavage sites. Identify the sequence DDDDK and cleave the peptide at the C-terminus of the lysine residue. This enzyme can cleave fusion proteins with recognition sequences. Enterokinase remains active under many reaction conditions and can effectively cleave fusion proteins over a wide pH range (4.5-9.5), a wide temperature range (4-45 ° C), and the presence of various detergents and denaturants.

Purity: ≥ 95% (SDS-PAGE)

Vitality definition: The amount of enzyme required to cleave 50 μ g of fusion protein at 16 ° C for 16-24 hours is one active unit.

Enzyme digestion conditions

Fusion protein :0.1mg
Recombinant enterokinase :2U
10x buffer :10μl
Temperature (ºC): 16
Enzyme digestion volume :100μl
Time (hours): 16-24

Example of results:

Company Profile

Nanjing Yanqiao Technology Co., Ltd is a modern high-tech chemical enterprise specializing in the R & D, production and sales of chemical reagents, biological reagents and fine chemical products.

Our company has various products, mainly engaged in various biochemical reagents, including enzymes, proteins, amino acids, carbohydrates, plant hormones, separation materials, culture media, buffers, pigments, chemical additives, etc.

The products are widely used in chemical plants, power plants, lubricating oil plants, pharmaceutical plants and water treatment processes, as well as the construction industry, agriculture, printing and dyeing industry, stationery production, university laboratories, scientific research institutions and other fields.

We adhere to the management principle of "quality first, service first, continuous improvement and innovation to meet customers", and take "zero defects, zero complaints" as the quality goal.

With mature and stable products, considerate service and good reputation, Nanjing Yanqiao Technology Co., Ltd. Sincerely welcomes friends at home and abroad to negotiate and cooperate, and work together!

Packaging & Shipping