T4 DNA Ligase; 9015-85-4; Factory Price; DNA; Experimental Reagents; T4

• Customization: Available
• CAS No.: 9015-85-4
• Formula: /
• Model NO.: YQEN0067
• EINECS: 232-770-0
• Classification: Biochemical Reagents
• Grade: Br
• Specific Usage: For Biological Purpose, Technical Grade, Pratical Use
• Content: 1000u/UL
• Usage: Laboratory Reagents, Teaching Reagents
• Source: /
• Habit Appellation: T4 DNA Ligase
• Application: Industry, Agriculture
• Property: Biochemical Reagent
• Appearance: Liquid
• Store: -20
• Enzyme Activity: 1000u/UL
• Purpose: Experimental Study
• Transport Package: Plastic Bottle
• Specification: 40ku
• Trademark: /
• Origin: China
• Production Capacity: 90000pieces/Year

Product Description

Product Description

 

Product Name	T4 DNA Ligase
CAS	9015-85-4
Purity (content)	≥ 1000u/ul
Code	YQEN0067
Packing	40ku

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English name: T4 DNA Ligase

CAS number: 9015-85-4

Grade: BR

Source: This T4 DNA Ligase is expressed in Escherichia coli, and the source of the expressed gene is T4 bacteriophage.

Activity definition: One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of lambda DNA in 30 min at 16 ºC in 20 μ l of the assay mixture containing 50 mM Tris, pH 7.5, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, 25 μ g/ml BSA, and a 5 '- DNA terminal concentration of 0.12 μ M (300 μ g/ml).

Enzyme storage solution: 20 mM Tris, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) glycol.

10X Ligation Buffer:400 mM Tris, pH 7.8, 100 mM MgCl2, 100 mM DTT, 5 mM ATP.

Inactivation or inhibition: Incubation at 65 ºC for 10 minutes can lead to the inactivation of T4 DNA ligase; When the concentration of NaCl or KCl is greater than 200mM, it strongly inhibits T4 DNA ligase.

Appearance: Liquid. This enzyme catalyzes the formation of phosphodiester bonds between adjacent 5 'phosphate groups and 3' - hydroxy terminals in double stranded DNA or RNA. It can also repair single stranded incisions in double stranded DNA, RNA or DNA/RNA complexes, connecting the sticky and flat ends of DNA, but this enzyme has no enzymatic activity against single stranded nucleic acid. This enzyme requires the cofactor ATP

Purpose: Biochemical research. Connection of sticky or flat end double stranded DNA; Double stranded oligonucleotide junction and double stranded DNA connection; Repair of gaps in double stranded DNA, RNA, or DNA-RNA complexes; Ligase mediated RNA detection; Site specific mutations; Amplified fragment length polymorphism

Storage: -20 ° C

Company Profile

Nanjing Yanqiao Technology Co., Ltd is a modern high-tech chemical enterprise specializing in the R & D, production and sales of chemical reagents, biological reagents and fine chemical products.

Our company has various products, mainly engaged in various biochemical reagents, including enzymes, proteins, amino acids, carbohydrates, plant hormones, separation materials, culture media, buffers, pigments, chemical additives, etc.

The products are widely used in chemical plants, power plants, lubricating oil plants, pharmaceutical plants and water treatment processes, as well as the construction industry, agriculture, printing and dyeing industry, stationery production, university laboratories, scientific research institutions and other fields.

We adhere to the management principle of "quality first, service first, continuous improvement and innovation to meet customers", and take "zero defects, zero complaints" as the quality goal.

With mature and stable products, considerate service and good reputation, Nanjing Yanqiao Technology Co., Ltd. Sincerely welcomes friends at home and abroad to negotiate and cooperate, and work together!

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