T7 Rna Polymerase; 9014-24-8; Polymerase; Factory Price; Experimental Reagents

• Customization: Available
• CAS No.: 9014-24-8
• Formula: /
• Model NO.: YQEN0066
• EINECS: 232-756-4
• Classification: Biochemical Reagents
• Grade: Br
• Specific Usage: For Biological Purpose, Technical Grade, Pratical Use
• Content: 20u/UL
• Usage: Laboratory Reagents, Teaching Reagents
• Source: /
• Habit Appellation: T7 Rna Polymerase
• Application: Industry, Agriculture
• Property: Biochemical Reagent
• Appearance: Buffered Aqueous Solution
• Store: -20
• Enzyme Activity: 20u/UL
• Purpose: Experimental Study
• Transport Package: Plastic Bottle
• Specification: 1ku; 5ku
• Trademark: /
• Origin: China
• Production Capacity: 90000pieces/Year

Product Description

Product Description

 

Product Name	T7 RNA Polymerase
CAS	9014-24-8
Purity (content)	≥ 20u/ul
Code	YQEN0066
Packing	1ku;5ku

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English name: T7 RNA Polymerase

This enzyme is derived from E. coli and expressed as the bacteriophage T7 RNA Polymerase gene. It is a highly specific DNA dependent 5 "→ 3" RNA polymerase that recognizes the T7 promoter sequence. T7 RNA Polymerase can catalyze the incorporation of NTP downstream of single or double stranded DNA T7 promoter, synthesizing RNA complementary to the template DNA downstream of T7 promoter.

Features: T7 RNA Polymerase can recognize modified NTPs, such as biotin labeled, d-i-g-o-x-i-n labeled, and fluorescein labeled NTPs, and can be used for the synthesis of various labeled RNAs. At the same time, it has high specificity for the T7 promoter.

Activity definition: The amount of enzyme required to catalyze the incorporation of 1 nmol AMP into polynucleotides within 60 minutes at 37 ºC is defined as 1 active unit.

Activity detection conditions: 40mM Tris HCl (pH 8.0), 6mM MgCl2, 10mM DTT, 2mM spermidine, 0.5mM NTP, 0.6MBq/ml [3H] - ATP, 20 μ g/ml plasma DNA containing the specific T7 RNA polymerase promoter sequence.

Purity: No DNA endonucleases and exonucleases, no RNA enzymes.

Enzyme storage solution: 50mM Tris HCl (pH 8.0), 150mM NaCl, 5mM DTT, 0.1mg/ml BSA, 0.5mM Elugent, 50% glycol.

Translation Buffer (5X): 200mM Tris HCl (pH 7.9 at 25 ºC), 30mM MgCl2, 50mM DTT, 50mM NaCl, 10mM spermidine.

Deactivation or inhibition: Heating at 70 ºC for 10 minutes can inactivate T7 RNA Polymerase. Adding an appropriate amount of EDTA can also inactivate T7 RNA Polymerase. Chelating agents, sodium, potassium, or ammonium salts with concentrations greater than 150mM can significantly inhibit the activity of T7 RNA Polymerase.

Usage: Used for RNA synthesis. The synthesized RNA can be used as a hybridization probe, genomic DNA sequence analysis, RNase protection assay, antisense RNA synthesis, as an RNA template for in vitro translation, as a substrate for RNA splicing research, RNA secondary structure and RNA protein interaction, nucleic acid amplification analysis, siRNA, miRNA, and other small RNAs.

Storage: -20 ºC

Company Profile

Nanjing Yanqiao Technology Co., Ltd is a modern high-tech chemical enterprise specializing in the R & D, production and sales of chemical reagents, biological reagents and fine chemical products.

Our company has various products, mainly engaged in various biochemical reagents, including enzymes, proteins, amino acids, carbohydrates, plant hormones, separation materials, culture media, buffers, pigments, chemical additives, etc.

The products are widely used in chemical plants, power plants, lubricating oil plants, pharmaceutical plants and water treatment processes, as well as the construction industry, agriculture, printing and dyeing industry, stationery production, university laboratories, scientific research institutions and other fields.

We adhere to the management principle of "quality first, service first, continuous improvement and innovation to meet customers", and take "zero defects, zero complaints" as the quality goal.

With mature and stable products, considerate service and good reputation, Nanjing Yanqiao Technology Co., Ltd. Sincerely welcomes friends at home and abroad to negotiate and cooperate, and work together!

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