Proteinase K From Tritirachium Album; 39450-01-6; Proteinase K

• Customization: Available
• CAS No.: 39450-01-6
• Formula: C29h27n2o12p
• Model NO.: YQEN0050
• Classification: Biochemical Reagents
• Grade: Br
• Specific Usage: For Biological Purpose, Technical Grade, Pratical Use
• Content: 30u/Mg Protein
• Usage: Laboratory Reagents, Teaching Reagents
• Source: Tritirachium Album
• Habit Appellation: Proteinase K
• Application: Industry, Agriculture
• Property: Biochemical Reagent
• Appearance: White Amorphous Powder
• Store: -20
• Enzyme Activity: 30u/Mg Protein
• Purpose: Experimental Study
• Molecular Weight: 29 Kda
• Isoelectric Point: 7.81
• Transport Package: Plastic Bottle
• Specification: 25mg; 100mg; 250mg; 500mg; 1g; 5g; 10g
• Trademark: /
• Origin: China
• Production Capacity: 90000pieces/Year

Product Description

Product Description

 

Product Name	Proteinase K
CAS	39450-01-6
Purity (content)	≥ 30U/mg protein
Code	YQEN0050
Packing	25mg; 100mg; 250mg; 500mg; 1g; 5g; 10g

Often used to degrade proteins and produce short peptides.

Protease K is an important serine protease secreted by Candida albicans. In 1974, Wolfgang Ebeling et al. isolated and purified protease K from the culture medium of Candida albicans using precipitation, ion exchange chromatography, and coagulation chromatography. Protease K belongs to the Bacillus subtilis family. It has high enzyme activity and wide substrate specificity, and is often used to degrade proteins and produce short peptides. It has the typical catalytic triad Asp39 His69 Ser224 characteristics unique to serine p-r-o-t-e-a-s-e-s, and two Ca2+binding sites around the active center increase its stability, allowing it to maintain high enzyme activity under a wider range of conditions.

Appearance: White amorphous freeze-dried powder
Specific activity : ≥ 30U/mg protein
No deoxyribonuclease activity detected
No ribonuclease activity detected

Enzymatic properties of protease K:
Source: Tritirachium album
Classification: EC 3.4.21.64
Molecular weight: 29 kDa (SDS-PAGE)
Isoelectric point: 7.81
Both pH 7.0 and 12.0 exhibit high activity
pH Stability : pH 4.5-12.5 (25 ºC, 16 h)
Thermal stability: Stable below 50 ºC (pH 8.0, 30 minutes)
Activator: SDS, urea
Inhibitors : Diisopropylfluorophosphate (DFIP), benzenesulfonyl fluoride (PMSF)
Storage conditions : The dry powder state can be stored for a long time at -20 ºC; After dissolution, it should be divided into appropriate volumes for short-term storage at 2-8 ºC and long-term storage at -20 ºC

Application:

1. Application in nucleic acid extraction and detection: Protease K can degrade histones tightly bound to nucleic acid, separate DNA from proteins, and purify DNA; Moreover, protease K can degrade RNA hydrolase activity (Rnase), inhibit the hydrolysis of template RNA by Rnase, and play an indispensable role in RNA extraction and detection.

2. Application in in situ hybridization: Protease K is commonly used to digest proteins around target DNA, facilitating probe penetration and improving detection sensitivity. It should be noted that high concentration of protease K or prolonged digestion time can damage the cell structure, leading to phenomena such as disappearance and detachment of the nucleus, affecting the binding of nucleic acids.

3. Application in non protein component extraction: Protease K can degrade protein impurities, separate target components from proteins from biological samples, improve product purity and production efficiency, especially for degrading harmful protein components such as endotoxins.

Company Profile

Nanjing Yanqiao Technology Co., Ltd is a modern high-tech chemical enterprise specializing in the R & D, production and sales of chemical reagents, biological reagents and fine chemical products.

Our company has various products, mainly engaged in various biochemical reagents, including enzymes, proteins, amino acids, carbohydrates, plant hormones, separation materials, culture media, buffers, pigments, chemical additives, etc.

The products are widely used in chemical plants, power plants, lubricating oil plants, pharmaceutical plants and water treatment processes, as well as the construction industry, agriculture, printing and dyeing industry, stationery production, university laboratories, scientific research institutions and other fields.

We adhere to the management principle of "quality first, service first, continuous improvement and innovation to meet customers", and take "zero defects, zero complaints" as the quality goal.

With mature and stable products, considerate service and good reputation, Nanjing Yanqiao Technology Co., Ltd. Sincerely welcomes friends at home and abroad to negotiate and cooperate, and work together!

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